Pollen Flow of Winter Triticale (x Triticosecale Wittmack) Investigated with Transgenic Line Expressing ?-Glucuronidase Gene

A transgenic winter triticale line expressing the uidA gene, encoding ?-glucuronidase, was used to assess pollen flow in field experiments over two consecutive vegetation seasons central Poland. The experimental design included variants of mixed and non-transgenic lines. Pollen grains were collected using passive traps located at 0, 10, 30, 60 85 m from line. GM detected histochemically by staining with x-Gluc. positive effect temperature increase, as well strength direction wind on number spread observed. Regardless experiment year variant, only few observed a distance m. In first study amount 300-fold lower than source 140-fold second year. times when surrounded line, compared an empty field. On basis obtained results, we suggest 100 for migration, although longer flight incidents are possible extreme atmospheric conditions.